recombinant igfbp5 (R&D Systems)
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recombinant igfbp5
Recombinant Igfbp5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+igfbp5/Recombinant+Mouse+IGFBP-5+Protein%2C+CF/pmc13050020-145-42-45
Average 94 stars, based on 5 article reviews
Recombinant Igfbp5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+igfbp5/Recombinant+Mouse+IGFBP-5+Protein%2C+CF/pmc13050020-145-42-45
Average 94 stars, based on 5 article reviews
recombinant igfbp5 - by Bioz Stars,
2026-11
94/100 stars
Images
1) Product Images from "Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult Mice"
Article Title: Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult Mice
Journal: The FASEB Journal
doi: 10.1096/fj.202500761RR
Figure Legend Snippet: Fetal testosterone supplementation rescues the adult kidney size by normalizing gene expression in the fetal kidney. (A) Anogenital distance of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (B) kidney weights of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (C) Igfbp5 expression (RNA‐seq) in E16.5 kidneys of WT, Hsd17b3 −/− (KO) and treated KO (res) groups (RPKM = Reads Per Kilobase Million). The lines indicate means. * = p ≤ 0.05. (D) Igfbp5 mRNA expression (qPCR) at different age points in WT kidneys. Points indicate means and whiskers SD. d = day, wk. = week. (E) Representative western blot images in E18.5 and quantification of IGFBP5 and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. * = p ≤ 0.05. (F) Immunofluorescent staining of IGFBP5 (red) and LRP2 (green) in E18.5 and 2‐week‐old WT and Hsd17b3 −/− (KO) kidneys. Scale bar: 50 μm.
Techniques Used: Gene Expression, Expressing, RNA Sequencing, Western Blot, Control, Staining
Figure Legend Snippet: IGFBP5 is likely regulated by AR and HNF4A, and in turn can affect cell proliferation. (A) Visualization of AR and HNF4A binding peaks and H3K4m1 and H3K27ac histone marks near Igfbp5 in ChIP‐seq data from Pihlajamaa et al., 2014. T = testosterone treatment. (B) Proliferation analysis of HEK 239 cells cultured with 0 (Ctrl), 25, 50, or 100 nM recombinant human IGFBP5. The lines indicate means. * = p ≤ 0.05. (C) Representative western blot images in E15.5 and quantification of phosphorylated AKT (pAKT), AKT, and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. (D) Representative western blot images in E15.5 and quantification of phosphorylated S6 ribosomal protein (pS6) and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. ** = p ≤ 0.01.
Techniques Used: Binding Assay, ChIP-sequencing, Cell Culture, Recombinant, Western Blot, Control
Related Articles
Recombinant:Article Title: Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5. Article Snippet: 20 Homocysteine (Hcy) is an independent risk factor of congenital heart disease (CHD), 21 but its exact underlying mechanism is unclear.. In this study, we collected amniotic 22 fluid (AF) supernatant samples from pregnant women carrying CHD-affected (n=16) 23 or normal (n=16) fetuses.. We found that Hcy concentrations were higher in the AF of 24 the CHD group when compared with normal pregnancies. Article Title: Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult Mice Article Snippet: For proliferation analysis, HEK 293 cell line was obtained from ATCC and tested negative for mycoplasma contamination. .. Cells were cultured on poly- L- lysine–coated plates (#P4832, SigmaAldrich) and switched to serum- free medium (DMEM/F12, #D2906, Sigma; 1% Penicillin/streptomycin, #A5256701, Gibco; 1% L- Glutamine, #25030–024, Gibco; 20 nM DHT) for 3 h before changing to fresh serum- free medium with 25, 50, or 100 nM Article Title: Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult Mice Article Snippet: For proliferation analysis, HEK 293 cell line was obtained from ATCC and tested negative for mycoplasma contamination. .. Cells were cultured on poly‐L‐lysine–coated plates (#P4832, Sigma‐Aldrich) and switched to serum‐free medium (DMEM/F12, #D2906, Sigma; 1% Penicillin/streptomycin, #A5256701, Gibco; 1% L‐Glutamine, #25030–024, Gibco; 20 nM DHT) for 3 h before changing to fresh serum‐free medium with 25, 50, or 100 nM Article Title: Secreted IGFBP5 Mediates mTORC1-Dependent Feedback Inhibition of IGF-1 Signaling Article Snippet: .. Intriguingly, depletion of IGFBP5 in TSC2 −/− MEFs greatly sensitizes them to IGF-1 stimulation ( ). fig ft0 fig mode=article f1 Figure 3 caption a4 IGFBP5 is a potent inhibitor of IGF-1 signaling. (A) Addition of Cell Culture:Article Title: Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult Mice Article Snippet: For proliferation analysis, HEK 293 cell line was obtained from ATCC and tested negative for mycoplasma contamination. .. Cells were cultured on poly- L- lysine–coated plates (#P4832, SigmaAldrich) and switched to serum- free medium (DMEM/F12, #D2906, Sigma; 1% Penicillin/streptomycin, #A5256701, Gibco; 1% L- Glutamine, #25030–024, Gibco; 20 nM DHT) for 3 h before changing to fresh serum- free medium with 25, 50, or 100 nM Article Title: Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult Mice Article Snippet: For proliferation analysis, HEK 293 cell line was obtained from ATCC and tested negative for mycoplasma contamination. .. Cells were cultured on poly‐L‐lysine–coated plates (#P4832, Sigma‐Aldrich) and switched to serum‐free medium (DMEM/F12, #D2906, Sigma; 1% Penicillin/streptomycin, #A5256701, Gibco; 1% L‐Glutamine, #25030–024, Gibco; 20 nM DHT) for 3 h before changing to fresh serum‐free medium with 25, 50, or 100 nM Activation Assay:Article Title: Secreted IGFBP5 Mediates mTORC1-Dependent Feedback Inhibition of IGF-1 Signaling Article Snippet: .. Intriguingly, depletion of IGFBP5 in TSC2 −/− MEFs greatly sensitizes them to IGF-1 stimulation ( ). fig ft0 fig mode=article f1 Figure 3 caption a4 IGFBP5 is a potent inhibitor of IGF-1 signaling. (A) Addition of Knockdown:Article Title: Secreted IGFBP5 Mediates mTORC1-Dependent Feedback Inhibition of IGF-1 Signaling Article Snippet: .. Intriguingly, depletion of IGFBP5 in TSC2 −/− MEFs greatly sensitizes them to IGF-1 stimulation ( ). fig ft0 fig mode=article f1 Figure 3 caption a4 IGFBP5 is a potent inhibitor of IGF-1 signaling. (A) Addition of Functional Assay:Article Title: Secreted IGFBP5 Mediates mTORC1-Dependent Feedback Inhibition of IGF-1 Signaling Article Snippet: .. Intriguingly, depletion of IGFBP5 in TSC2 −/− MEFs greatly sensitizes them to IGF-1 stimulation ( ). fig ft0 fig mode=article f1 Figure 3 caption a4 IGFBP5 is a potent inhibitor of IGF-1 signaling. (A) Addition of |