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recombinant igfbp5  (R&D Systems)


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    R&D Systems recombinant igfbp5
    Fetal testosterone supplementation rescues the adult kidney size by normalizing gene expression in the fetal kidney. (A) Anogenital distance of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (B) kidney weights of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (C) <t>Igfbp5</t> expression (RNA‐seq) in E16.5 kidneys of WT, Hsd17b3 −/− (KO) and treated KO (res) groups (RPKM = Reads Per Kilobase Million). The lines indicate means. * = p ≤ 0.05. (D) Igfbp5 mRNA expression (qPCR) at different age points in WT kidneys. Points indicate means and whiskers SD. d = day, wk. = week. (E) Representative western blot images in E18.5 and quantification of IGFBP5 and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. * = p ≤ 0.05. (F) Immunofluorescent staining of IGFBP5 (red) and LRP2 (green) in E18.5 and 2‐week‐old WT and Hsd17b3 −/− (KO) kidneys. Scale bar: 50 μm.
    Recombinant Igfbp5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+igfbp5/pmc13050020-145-42-45?v=R%26D+Systems
    Average 94 stars, based on 4 article reviews
    recombinant igfbp5 - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult Mice"

    Article Title: Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult Mice

    Journal: The FASEB Journal

    doi: 10.1096/fj.202500761RR

    Fetal testosterone supplementation rescues the adult kidney size by normalizing gene expression in the fetal kidney. (A) Anogenital distance of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (B) kidney weights of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (C) Igfbp5 expression (RNA‐seq) in E16.5 kidneys of WT, Hsd17b3 −/− (KO) and treated KO (res) groups (RPKM = Reads Per Kilobase Million). The lines indicate means. * = p ≤ 0.05. (D) Igfbp5 mRNA expression (qPCR) at different age points in WT kidneys. Points indicate means and whiskers SD. d = day, wk. = week. (E) Representative western blot images in E18.5 and quantification of IGFBP5 and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. * = p ≤ 0.05. (F) Immunofluorescent staining of IGFBP5 (red) and LRP2 (green) in E18.5 and 2‐week‐old WT and Hsd17b3 −/− (KO) kidneys. Scale bar: 50 μm.
    Figure Legend Snippet: Fetal testosterone supplementation rescues the adult kidney size by normalizing gene expression in the fetal kidney. (A) Anogenital distance of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (B) kidney weights of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (C) Igfbp5 expression (RNA‐seq) in E16.5 kidneys of WT, Hsd17b3 −/− (KO) and treated KO (res) groups (RPKM = Reads Per Kilobase Million). The lines indicate means. * = p ≤ 0.05. (D) Igfbp5 mRNA expression (qPCR) at different age points in WT kidneys. Points indicate means and whiskers SD. d = day, wk. = week. (E) Representative western blot images in E18.5 and quantification of IGFBP5 and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. * = p ≤ 0.05. (F) Immunofluorescent staining of IGFBP5 (red) and LRP2 (green) in E18.5 and 2‐week‐old WT and Hsd17b3 −/− (KO) kidneys. Scale bar: 50 μm.

    Techniques Used: Gene Expression, Expressing, RNA Sequencing, Western Blot, Control, Staining

    IGFBP5 is likely regulated by AR and HNF4A, and in turn can affect cell proliferation. (A) Visualization of AR and HNF4A binding peaks and H3K4m1 and H3K27ac histone marks near Igfbp5 in ChIP‐seq data from Pihlajamaa et al., 2014. T = testosterone treatment. (B) Proliferation analysis of HEK 239 cells cultured with 0 (Ctrl), 25, 50, or 100 nM recombinant human IGFBP5. The lines indicate means. * = p ≤ 0.05. (C) Representative western blot images in E15.5 and quantification of phosphorylated AKT (pAKT), AKT, and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. (D) Representative western blot images in E15.5 and quantification of phosphorylated S6 ribosomal protein (pS6) and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. ** = p ≤ 0.01.
    Figure Legend Snippet: IGFBP5 is likely regulated by AR and HNF4A, and in turn can affect cell proliferation. (A) Visualization of AR and HNF4A binding peaks and H3K4m1 and H3K27ac histone marks near Igfbp5 in ChIP‐seq data from Pihlajamaa et al., 2014. T = testosterone treatment. (B) Proliferation analysis of HEK 239 cells cultured with 0 (Ctrl), 25, 50, or 100 nM recombinant human IGFBP5. The lines indicate means. * = p ≤ 0.05. (C) Representative western blot images in E15.5 and quantification of phosphorylated AKT (pAKT), AKT, and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. (D) Representative western blot images in E15.5 and quantification of phosphorylated S6 ribosomal protein (pS6) and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. ** = p ≤ 0.01.

    Techniques Used: Binding Assay, ChIP-sequencing, Cell Culture, Recombinant, Western Blot, Control



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    Image Search Results


    Fetal testosterone supplementation rescues the adult kidney size by normalizing gene expression in the fetal kidney. (A) Anogenital distance of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (B) kidney weights of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (C) Igfbp5 expression (RNA‐seq) in E16.5 kidneys of WT, Hsd17b3 −/− (KO) and treated KO (res) groups (RPKM = Reads Per Kilobase Million). The lines indicate means. * = p ≤ 0.05. (D) Igfbp5 mRNA expression (qPCR) at different age points in WT kidneys. Points indicate means and whiskers SD. d = day, wk. = week. (E) Representative western blot images in E18.5 and quantification of IGFBP5 and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. * = p ≤ 0.05. (F) Immunofluorescent staining of IGFBP5 (red) and LRP2 (green) in E18.5 and 2‐week‐old WT and Hsd17b3 −/− (KO) kidneys. Scale bar: 50 μm.

    Journal: The FASEB Journal

    Article Title: Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult Mice

    doi: 10.1096/fj.202500761RR

    Figure Lengend Snippet: Fetal testosterone supplementation rescues the adult kidney size by normalizing gene expression in the fetal kidney. (A) Anogenital distance of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (B) kidney weights of 3‐month‐old Hsd17b3 −/− (KO) and WT males without and with testosterone supplementation (res) at E14.5–17.5. The lines indicate means. * = p ≤ 0.05 ** = p ≤ 0.01. (C) Igfbp5 expression (RNA‐seq) in E16.5 kidneys of WT, Hsd17b3 −/− (KO) and treated KO (res) groups (RPKM = Reads Per Kilobase Million). The lines indicate means. * = p ≤ 0.05. (D) Igfbp5 mRNA expression (qPCR) at different age points in WT kidneys. Points indicate means and whiskers SD. d = day, wk. = week. (E) Representative western blot images in E18.5 and quantification of IGFBP5 and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. * = p ≤ 0.05. (F) Immunofluorescent staining of IGFBP5 (red) and LRP2 (green) in E18.5 and 2‐week‐old WT and Hsd17b3 −/− (KO) kidneys. Scale bar: 50 μm.

    Article Snippet: Cells were cultured on poly‐L‐lysine–coated plates (#P4832, Sigma‐Aldrich) and switched to serum‐free medium (DMEM/F12, #D2906, Sigma; 1% Penicillin/streptomycin, #A5256701, Gibco; 1% L‐Glutamine, #25030–024, Gibco; 20 nM DHT) for 3 h before changing to fresh serum‐free medium with 25, 50, or 100 nM recombinant IGFBP5 (#578‐B5, R&D Systems).

    Techniques: Gene Expression, Expressing, RNA Sequencing, Western Blot, Control, Staining

    IGFBP5 is likely regulated by AR and HNF4A, and in turn can affect cell proliferation. (A) Visualization of AR and HNF4A binding peaks and H3K4m1 and H3K27ac histone marks near Igfbp5 in ChIP‐seq data from Pihlajamaa et al., 2014. T = testosterone treatment. (B) Proliferation analysis of HEK 239 cells cultured with 0 (Ctrl), 25, 50, or 100 nM recombinant human IGFBP5. The lines indicate means. * = p ≤ 0.05. (C) Representative western blot images in E15.5 and quantification of phosphorylated AKT (pAKT), AKT, and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. (D) Representative western blot images in E15.5 and quantification of phosphorylated S6 ribosomal protein (pS6) and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. ** = p ≤ 0.01.

    Journal: The FASEB Journal

    Article Title: Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult Mice

    doi: 10.1096/fj.202500761RR

    Figure Lengend Snippet: IGFBP5 is likely regulated by AR and HNF4A, and in turn can affect cell proliferation. (A) Visualization of AR and HNF4A binding peaks and H3K4m1 and H3K27ac histone marks near Igfbp5 in ChIP‐seq data from Pihlajamaa et al., 2014. T = testosterone treatment. (B) Proliferation analysis of HEK 239 cells cultured with 0 (Ctrl), 25, 50, or 100 nM recombinant human IGFBP5. The lines indicate means. * = p ≤ 0.05. (C) Representative western blot images in E15.5 and quantification of phosphorylated AKT (pAKT), AKT, and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. (D) Representative western blot images in E15.5 and quantification of phosphorylated S6 ribosomal protein (pS6) and loading control β‐actin in E15.5 and E18.5 WT and Hsd17b3 −/− (KO) kidney homogenates. The lines indicate means. ** = p ≤ 0.01.

    Article Snippet: Cells were cultured on poly‐L‐lysine–coated plates (#P4832, Sigma‐Aldrich) and switched to serum‐free medium (DMEM/F12, #D2906, Sigma; 1% Penicillin/streptomycin, #A5256701, Gibco; 1% L‐Glutamine, #25030–024, Gibco; 20 nM DHT) for 3 h before changing to fresh serum‐free medium with 25, 50, or 100 nM recombinant IGFBP5 (#578‐B5, R&D Systems).

    Techniques: Binding Assay, ChIP-sequencing, Cell Culture, Recombinant, Western Blot, Control